ecgm mv medium Search Results


99
PromoCell endothelial cell growth medium ecgm
Endothelial Cell Growth Medium Ecgm, supplied by PromoCell, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PromoCell endothelial cell growth medium mv
Passage of ranibizumab through the NDN or SHN did not change its typical characteristics. After incubation of VEGF-A 165 with ( A ) untreated ranibizumab or ( B ) ranibizumab repeatedly passed through a needle, free growth factor was determined by ELISA. Data from independent experiments were pooled and analyzed as described in Material and Methods, and are shown as scatter plots with means ± standard deviations. *p<0.05, **p<0.01, ***p<0.001, ****) p<0.0001; only statistically significant differences are shown. All measured concentrations of free VEGF-A were very low. ( C – F ) Confluent monolayers of retinal <t>endothelial</t> cells (iBREC) were exposed to vehicle (n=6), VEGF-A 165 (n=8), the growth factor plus ( C and F ) untreated (n=8), ( D and F ) SHN-treated (n=12) or ( E and F ) NDN-treated (n=11) ranibizumab for three days and the cell index was measured continuously. Cell index values were normalized and analyzed as described in Material and Methods. Means ± standard deviations are shown in ( C – E ), means only in ( F ). Ranibizumab efficiently prevented VEGF-A 165 -induced decline of cell index values which was not changed by its passage either through a SHN or the NDN.
Endothelial Cell Growth Medium Mv, supplied by PromoCell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PromoCell ecgm 2 medium
Passage of ranibizumab through the NDN or SHN did not change its typical characteristics. After incubation of VEGF-A 165 with ( A ) untreated ranibizumab or ( B ) ranibizumab repeatedly passed through a needle, free growth factor was determined by ELISA. Data from independent experiments were pooled and analyzed as described in Material and Methods, and are shown as scatter plots with means ± standard deviations. *p<0.05, **p<0.01, ***p<0.001, ****) p<0.0001; only statistically significant differences are shown. All measured concentrations of free VEGF-A were very low. ( C – F ) Confluent monolayers of retinal <t>endothelial</t> cells (iBREC) were exposed to vehicle (n=6), VEGF-A 165 (n=8), the growth factor plus ( C and F ) untreated (n=8), ( D and F ) SHN-treated (n=12) or ( E and F ) NDN-treated (n=11) ranibizumab for three days and the cell index was measured continuously. Cell index values were normalized and analyzed as described in Material and Methods. Means ± standard deviations are shown in ( C – E ), means only in ( F ). Ranibizumab efficiently prevented VEGF-A 165 -induced decline of cell index values which was not changed by its passage either through a SHN or the NDN.
Ecgm 2 Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Lonza egm-2 mv microvascular endothelial cell growth medium-2 bulletkit
Passage of ranibizumab through the NDN or SHN did not change its typical characteristics. After incubation of VEGF-A 165 with ( A ) untreated ranibizumab or ( B ) ranibizumab repeatedly passed through a needle, free growth factor was determined by ELISA. Data from independent experiments were pooled and analyzed as described in Material and Methods, and are shown as scatter plots with means ± standard deviations. *p<0.05, **p<0.01, ***p<0.001, ****) p<0.0001; only statistically significant differences are shown. All measured concentrations of free VEGF-A were very low. ( C – F ) Confluent monolayers of retinal <t>endothelial</t> cells (iBREC) were exposed to vehicle (n=6), VEGF-A 165 (n=8), the growth factor plus ( C and F ) untreated (n=8), ( D and F ) SHN-treated (n=12) or ( E and F ) NDN-treated (n=11) ranibizumab for three days and the cell index was measured continuously. Cell index values were normalized and analyzed as described in Material and Methods. Means ± standard deviations are shown in ( C – E ), means only in ( F ). Ranibizumab efficiently prevented VEGF-A 165 -induced decline of cell index values which was not changed by its passage either through a SHN or the NDN.
Egm 2 Mv Microvascular Endothelial Cell Growth Medium 2 Bulletkit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
PromoCell basal medium mv
Passage of ranibizumab through the NDN or SHN did not change its typical characteristics. After incubation of VEGF-A 165 with ( A ) untreated ranibizumab or ( B ) ranibizumab repeatedly passed through a needle, free growth factor was determined by ELISA. Data from independent experiments were pooled and analyzed as described in Material and Methods, and are shown as scatter plots with means ± standard deviations. *p<0.05, **p<0.01, ***p<0.001, ****) p<0.0001; only statistically significant differences are shown. All measured concentrations of free VEGF-A were very low. ( C – F ) Confluent monolayers of retinal <t>endothelial</t> cells (iBREC) were exposed to vehicle (n=6), VEGF-A 165 (n=8), the growth factor plus ( C and F ) untreated (n=8), ( D and F ) SHN-treated (n=12) or ( E and F ) NDN-treated (n=11) ranibizumab for three days and the cell index was measured continuously. Cell index values were normalized and analyzed as described in Material and Methods. Means ± standard deviations are shown in ( C – E ), means only in ( F ). Ranibizumab efficiently prevented VEGF-A 165 -induced decline of cell index values which was not changed by its passage either through a SHN or the NDN.
Basal Medium Mv, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PromoCell ecgm
Passage of ranibizumab through the NDN or SHN did not change its typical characteristics. After incubation of VEGF-A 165 with ( A ) untreated ranibizumab or ( B ) ranibizumab repeatedly passed through a needle, free growth factor was determined by ELISA. Data from independent experiments were pooled and analyzed as described in Material and Methods, and are shown as scatter plots with means ± standard deviations. *p<0.05, **p<0.01, ***p<0.001, ****) p<0.0001; only statistically significant differences are shown. All measured concentrations of free VEGF-A were very low. ( C – F ) Confluent monolayers of retinal <t>endothelial</t> cells (iBREC) were exposed to vehicle (n=6), VEGF-A 165 (n=8), the growth factor plus ( C and F ) untreated (n=8), ( D and F ) SHN-treated (n=12) or ( E and F ) NDN-treated (n=11) ranibizumab for three days and the cell index was measured continuously. Cell index values were normalized and analyzed as described in Material and Methods. Means ± standard deviations are shown in ( C – E ), means only in ( F ). Ranibizumab efficiently prevented VEGF-A 165 -induced decline of cell index values which was not changed by its passage either through a SHN or the NDN.
Ecgm, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Lonza microvascular endothelial cell growth medium-2 (egm-2mv)
Passage of ranibizumab through the NDN or SHN did not change its typical characteristics. After incubation of VEGF-A 165 with ( A ) untreated ranibizumab or ( B ) ranibizumab repeatedly passed through a needle, free growth factor was determined by ELISA. Data from independent experiments were pooled and analyzed as described in Material and Methods, and are shown as scatter plots with means ± standard deviations. *p<0.05, **p<0.01, ***p<0.001, ****) p<0.0001; only statistically significant differences are shown. All measured concentrations of free VEGF-A were very low. ( C – F ) Confluent monolayers of retinal <t>endothelial</t> cells (iBREC) were exposed to vehicle (n=6), VEGF-A 165 (n=8), the growth factor plus ( C and F ) untreated (n=8), ( D and F ) SHN-treated (n=12) or ( E and F ) NDN-treated (n=11) ranibizumab for three days and the cell index was measured continuously. Cell index values were normalized and analyzed as described in Material and Methods. Means ± standard deviations are shown in ( C – E ), means only in ( F ). Ranibizumab efficiently prevented VEGF-A 165 -induced decline of cell index values which was not changed by its passage either through a SHN or the NDN.
Microvascular Endothelial Cell Growth Medium 2 (Egm 2mv), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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98
PromoCell endothelial cell growth medium mv 2 media
Passage of ranibizumab through the NDN or SHN did not change its typical characteristics. After incubation of VEGF-A 165 with ( A ) untreated ranibizumab or ( B ) ranibizumab repeatedly passed through a needle, free growth factor was determined by ELISA. Data from independent experiments were pooled and analyzed as described in Material and Methods, and are shown as scatter plots with means ± standard deviations. *p<0.05, **p<0.01, ***p<0.001, ****) p<0.0001; only statistically significant differences are shown. All measured concentrations of free VEGF-A were very low. ( C – F ) Confluent monolayers of retinal <t>endothelial</t> cells (iBREC) were exposed to vehicle (n=6), VEGF-A 165 (n=8), the growth factor plus ( C and F ) untreated (n=8), ( D and F ) SHN-treated (n=12) or ( E and F ) NDN-treated (n=11) ranibizumab for three days and the cell index was measured continuously. Cell index values were normalized and analyzed as described in Material and Methods. Means ± standard deviations are shown in ( C – E ), means only in ( F ). Ranibizumab efficiently prevented VEGF-A 165 -induced decline of cell index values which was not changed by its passage either through a SHN or the NDN.
Endothelial Cell Growth Medium Mv 2 Media, supplied by PromoCell, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
PromoCell ecgm modecgm medium
Passage of ranibizumab through the NDN or SHN did not change its typical characteristics. After incubation of VEGF-A 165 with ( A ) untreated ranibizumab or ( B ) ranibizumab repeatedly passed through a needle, free growth factor was determined by ELISA. Data from independent experiments were pooled and analyzed as described in Material and Methods, and are shown as scatter plots with means ± standard deviations. *p<0.05, **p<0.01, ***p<0.001, ****) p<0.0001; only statistically significant differences are shown. All measured concentrations of free VEGF-A were very low. ( C – F ) Confluent monolayers of retinal <t>endothelial</t> cells (iBREC) were exposed to vehicle (n=6), VEGF-A 165 (n=8), the growth factor plus ( C and F ) untreated (n=8), ( D and F ) SHN-treated (n=12) or ( E and F ) NDN-treated (n=11) ranibizumab for three days and the cell index was measured continuously. Cell index values were normalized and analyzed as described in Material and Methods. Means ± standard deviations are shown in ( C – E ), means only in ( F ). Ranibizumab efficiently prevented VEGF-A 165 -induced decline of cell index values which was not changed by its passage either through a SHN or the NDN.
Ecgm Modecgm Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
PromoCell ec growth medium ecgm
Passage of ranibizumab through the NDN or SHN did not change its typical characteristics. After incubation of VEGF-A 165 with ( A ) untreated ranibizumab or ( B ) ranibizumab repeatedly passed through a needle, free growth factor was determined by ELISA. Data from independent experiments were pooled and analyzed as described in Material and Methods, and are shown as scatter plots with means ± standard deviations. *p<0.05, **p<0.01, ***p<0.001, ****) p<0.0001; only statistically significant differences are shown. All measured concentrations of free VEGF-A were very low. ( C – F ) Confluent monolayers of retinal <t>endothelial</t> cells (iBREC) were exposed to vehicle (n=6), VEGF-A 165 (n=8), the growth factor plus ( C and F ) untreated (n=8), ( D and F ) SHN-treated (n=12) or ( E and F ) NDN-treated (n=11) ranibizumab for three days and the cell index was measured continuously. Cell index values were normalized and analyzed as described in Material and Methods. Means ± standard deviations are shown in ( C – E ), means only in ( F ). Ranibizumab efficiently prevented VEGF-A 165 -induced decline of cell index values which was not changed by its passage either through a SHN or the NDN.
Ec Growth Medium Ecgm, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PromoCell ecgm mv
Passage of ranibizumab through the NDN or SHN did not change its typical characteristics. After incubation of VEGF-A 165 with ( A ) untreated ranibizumab or ( B ) ranibizumab repeatedly passed through a needle, free growth factor was determined by ELISA. Data from independent experiments were pooled and analyzed as described in Material and Methods, and are shown as scatter plots with means ± standard deviations. *p<0.05, **p<0.01, ***p<0.001, ****) p<0.0001; only statistically significant differences are shown. All measured concentrations of free VEGF-A were very low. ( C – F ) Confluent monolayers of retinal <t>endothelial</t> cells (iBREC) were exposed to vehicle (n=6), VEGF-A 165 (n=8), the growth factor plus ( C and F ) untreated (n=8), ( D and F ) SHN-treated (n=12) or ( E and F ) NDN-treated (n=11) ranibizumab for three days and the cell index was measured continuously. Cell index values were normalized and analyzed as described in Material and Methods. Means ± standard deviations are shown in ( C – E ), means only in ( F ). Ranibizumab efficiently prevented VEGF-A 165 -induced decline of cell index values which was not changed by its passage either through a SHN or the NDN.
Ecgm Mv, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PromoCell cell culture medium
Passage of ranibizumab through the NDN or SHN did not change its typical characteristics. After incubation of VEGF-A 165 with ( A ) untreated ranibizumab or ( B ) ranibizumab repeatedly passed through a needle, free growth factor was determined by ELISA. Data from independent experiments were pooled and analyzed as described in Material and Methods, and are shown as scatter plots with means ± standard deviations. *p<0.05, **p<0.01, ***p<0.001, ****) p<0.0001; only statistically significant differences are shown. All measured concentrations of free VEGF-A were very low. ( C – F ) Confluent monolayers of retinal <t>endothelial</t> cells (iBREC) were exposed to vehicle (n=6), VEGF-A 165 (n=8), the growth factor plus ( C and F ) untreated (n=8), ( D and F ) SHN-treated (n=12) or ( E and F ) NDN-treated (n=11) ranibizumab for three days and the cell index was measured continuously. Cell index values were normalized and analyzed as described in Material and Methods. Means ± standard deviations are shown in ( C – E ), means only in ( F ). Ranibizumab efficiently prevented VEGF-A 165 -induced decline of cell index values which was not changed by its passage either through a SHN or the NDN.
Cell Culture Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Passage of ranibizumab through the NDN or SHN did not change its typical characteristics. After incubation of VEGF-A 165 with ( A ) untreated ranibizumab or ( B ) ranibizumab repeatedly passed through a needle, free growth factor was determined by ELISA. Data from independent experiments were pooled and analyzed as described in Material and Methods, and are shown as scatter plots with means ± standard deviations. *p<0.05, **p<0.01, ***p<0.001, ****) p<0.0001; only statistically significant differences are shown. All measured concentrations of free VEGF-A were very low. ( C – F ) Confluent monolayers of retinal endothelial cells (iBREC) were exposed to vehicle (n=6), VEGF-A 165 (n=8), the growth factor plus ( C and F ) untreated (n=8), ( D and F ) SHN-treated (n=12) or ( E and F ) NDN-treated (n=11) ranibizumab for three days and the cell index was measured continuously. Cell index values were normalized and analyzed as described in Material and Methods. Means ± standard deviations are shown in ( C – E ), means only in ( F ). Ranibizumab efficiently prevented VEGF-A 165 -induced decline of cell index values which was not changed by its passage either through a SHN or the NDN.

Journal: Clinical Ophthalmology (Auckland, N.Z.)

Article Title: Novel Needle for Intravitreal Injections Does Not Affect Biological Activity of Anti-VEGF Drugs

doi: 10.2147/OPTH.S557508

Figure Lengend Snippet: Passage of ranibizumab through the NDN or SHN did not change its typical characteristics. After incubation of VEGF-A 165 with ( A ) untreated ranibizumab or ( B ) ranibizumab repeatedly passed through a needle, free growth factor was determined by ELISA. Data from independent experiments were pooled and analyzed as described in Material and Methods, and are shown as scatter plots with means ± standard deviations. *p<0.05, **p<0.01, ***p<0.001, ****) p<0.0001; only statistically significant differences are shown. All measured concentrations of free VEGF-A were very low. ( C – F ) Confluent monolayers of retinal endothelial cells (iBREC) were exposed to vehicle (n=6), VEGF-A 165 (n=8), the growth factor plus ( C and F ) untreated (n=8), ( D and F ) SHN-treated (n=12) or ( E and F ) NDN-treated (n=11) ranibizumab for three days and the cell index was measured continuously. Cell index values were normalized and analyzed as described in Material and Methods. Means ± standard deviations are shown in ( C – E ), means only in ( F ). Ranibizumab efficiently prevented VEGF-A 165 -induced decline of cell index values which was not changed by its passage either through a SHN or the NDN.

Article Snippet: Briefly, cells were cultured in Endothelial Cell Growth Medium MV (ECGM; # C22120 , PromoCell, Heidelberg, Germany) until a confluent cell monolayer was reached three days later, indicated by a constantly high cell index (CI ~ 20).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay